DNA-free PCR Reagents

 
MolTaq 16S/18S and Mastermix 16S/18S  
 
Benefits:
DNAfree Mastermix
  • Microbial DNA-free
  • Highly active Taq Polymerases
  • Ultra-sensitive detection of bacterial & fungal DNA
  • Suitable for Real-time PCR
  • High amplification activity for 40 cycles

There is a strong risk of false positive signals as a cause of amplification from contaminating DNA in PCR reagents, including the polymerising enzyme, primers and dNTPs.

Molzym’s purification technology removes traces of DNA from the PCR reagents and supplies a highly active enzyme in the products. This results in enhanced sensitivity and reliability of bacteria and fungi detection in the assays.

Solutions are offered to meet your requirements ranging from DNA-free Taq polymerases, MolTaq 16S/18S and Hot MolTaq 16S/18S to master mixes in its various designs, Mastermix 16S and Mastermix 18S kits. Assays are available, Mastermix 16S Primer and Mastermix 16S Complete, for the detection of bacterial DNA, and Mastermix 18S Primer and Mastermix 18S Complete, for the detection of fungal DNA by the amplification of a highly variable regions of the 16S and 18S rRNA gene, respectively.

If you want to use your own primers, we offer Mastermix 16S/18S Basic and Mastermix 16S/18S Dye kits which contain all components for the PCR reaction except primers.

Sequencing of amplicons combined with online homology BLAST search is an option for the identification of bacteria and fungi detected by Molzym assays, Mastermix16S/18S Complete or Mastermix 16S/18S Primer. Molzym offers sequencing primers that allow the sequence analysis and identification of bacteria and fungi. The primers bind to conserved regions within the amplified DNA enabling sequencing of highly discriminative sequences for strain identification. Sequencing primer SeqGP16 covers a variety of Gram-positive organisms, including staphylococci, streptococci and enterococci. Sequencing primer SeqGN16 on the other hand allows the identification of many pathogenic Gram-negative bacteria, including enterobacteria and pseudomonads. For more detailed list of strains see the FAQ.

Sequences generated by using the sequencing primers can be easily analysed by referring to our free sequence alignment software, SepsiTest BLAST. If you like to know more about this software, please click here.

 

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