PCR Amplification

Kits - DNA-free PCR Reagents

Mastermix16SComplete2    A series of kits are especially dedicated to the sensitive and specific detection of bacterial and fungal pathogens in samples. All reagents are manufactured DNA-free with respect to microbial DNA contaminations. Likewise, the reagents are highly active in their amplification performance. DNA-free MolTaq 16S/18S and Hot MolTaq 16S/18S are the Taq DNA polymerases of choice if you experience contamination problems with other standard Taqs. The new developed Hot MolTaq 16S/18S, combines the favourable characters of MolTaq 16S/18S with hot start amplification and thereby increased specificity.  Master mixes contain pre-assembled reagents necessary for PCR, including dNTPs, buffer, Mg2+ (3mM final) and BSA. Mastermix 16S/18S Basic and Mastermix 16S/18S Dye are available for assays using custom primers, Mastermix 16S Primer and Mastermix 16S Complete for universal detection of bacterial DNA, and Mastermix 18S Primer and Mastermix 18S Complete for universal detection of fungal DNA in samples by PCR and Real-Time PCR, respectively. The master mixes are 2.5x concentrated.

 

Product Overview:

Product Application Quotation Request
MolTaq 16S/18S Taq for bacterial and fungal DNA detection request
Hot MolTaq 16S/18S Hot start Taq for bacterial and fungal DNA detection
Mastermix 16S/18S Basic custom PCR assays
Mastermix 16S/18S Dye custom Real-Time PCR assays
Mastermix 16S Primer eubacterial PCR assay
Mastermix 18S Primer panfungal PCR assay
Mastermix 16S Complete eubacterial Real-Time PCR assay
Mastermix 18S Complete panfungal Real-Time PCR assay

 

 

Application Custom primers Eubacterial assays
Panfungal assays
Product

Mastermix 16S/18S Mastermix 16S Mastermix 18S
Basic Dye Primer
Complete
Primer
Complete
MolTaq 16S/18S, buffer, water + + + + + +
dNTPs + + + + + +
Fluorescing dye - + - + - +
Universal 16S primers - - + + - -
Universal 18S primers - - - - + +

 

 

DNA-free water is high quality PCR-grade water that is used in the setup of PCR and Real-Time PCR assays demanding high accuracy of analysis. Applications comprise quality control of manufacturing processes in pharma and biotechnology, contamination control of food products, environmental analysis, molecular infection diagnosis, SNP and other hereditary mutation analyses, and research. DNA-free water allows the precise molecular analysis with utmost reliability. This product is manufactured under strict quality control management guaranteeing the absence of human and microbial DNA contamination (<3% false-positive rate).

Product Content Quotation Request
DNA-free water, PCR-grade DNA-free water, 10x 1.7ml  request

 

Sequencing Primers in conjunction with eubacterial PCR assays (Mastermix 16S Primer and Mastermix 16S Complete) and panfungal PCR assays (Mastermix 18S Primer and Mastermix 18S Complete):

Product Content Application Quotation Request
Set of sequencing primers eubacterial SeqGP16 and SeqGN16 (10pmol/µl) sequencing of amplicons request
Sequencing primer panfungal SeqYeast (10pmol/µl) sequencing of amplicons

 

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Product Flyer

 

 

Kits - Standard PCR Reagents

MolTaq 

A variety of kits are available to meet your needs for high performance PCR amplification in daily routine. MolTaq is the standard Taq DNA polymerase kit supplying PCR buffer (1.5mM MgSO4) and PCR enhancer for high G+C content targets. MolTaq Basic enables optimization of PCR amplification by variation of Mg2+ concentration (basic buffer and extra 100 mM MgCl2 solution). Control of accurate pipetting and direct transfer of the amplification reaction to the gel for analysis is possible using MolTaqRED and MolTaqRED basic, respectively.  

 

Product Overview:

  Product Content Quotation Request
MolTaq Taq DNA polymerase, buffer (1.5mM MgSO4 final), PCR enhancer request
MolTaq basic Taq DNA polymerase, buffer, 100mM MgCl2, PCR enhancer
MolTaqRED Taq DNA polymerase, buffer (1.5mM MgSO4 final), PCR enhancer, red dye
MolTaqRED basic Taq DNA polymerase, buffer, 100mM MgCl2, PCR enhancer, red dye
Hot MolTaq Hot start Taq DNA polymerase, buffer (1.5mM MgSO4 final), PCR enhancer
Hot MolTaq basic       Hot start Taq DNA polymerase, 100mM MgSO4, PCR enhancer

 

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References - Standard PCR Reagents

Heim G, Walsh AM, Sweeney T, Doyle DN, O'Shea CJ, Ryan MT, O'Doherty JV (2014). Effect of seaweed-derived laminarin and fucoidan and zinc oxide on gut morphology, nutrient transporters, nutrient digestibility, growth performance and selected microbial populations in weaned pigs. Brit J Nutr 111, 1577-1585.

Krebes L, Zeidler L, Frankowski J, Bastrop R (2014) (Cryptic) sex in the microsporidian Nosema granulosis – evidence from parasite rDNA and host mitochondrial DNA. Inf Gen Evol 21, 259–268.

Voigt O, Herzog B, Jakobshagen A, Pöggeler S (2014) Autophagic kinases SmVPS34 and SmVPS15 are required for viability in the filamentous ascomycete Sordaria macrospora. Microbiol Res 169, 128–138.

Habel JC, Mulwa RK, Gassert F, Rödder D, Ulrich W, Borghesio L, Husemann M, Lens L (2014) Heredity, doi:10.1038/hdy.2014.15.

Weilandt R, Paulmann D, Schlottau K, Vallbracht A, Dotzauer A (2014) Mutational modifications of hepatitis A virus proteins 2B and 2C described for cell culture-adapted and attenuated virus are present in wild-type virus populations. Arch Virol, DOI: 10.1007/s00705-014-2103-6.

Schwentner M, Timms BV, Richter S (2013) Evolutionary systematics of the Australian Eocyzicus fauna (Crustacea: Branchiopoda: Spinicaudata) reveals hidden diversity and phylogeographic structure. J Zool Sys Evol Res. DOI: 1111/jzs.12038.

Stoll C, Sidhu J, Tiehm A, Toze S (2012) Prevalence of clinically relevant antibiotic resistance genes in surface water samples collected from Germany and Australia. Environ Sci Technol. DOI:10.1021/es302020s.

Sergouniotis PI, Sohn EH, Li Z at al. (2011) Phenotypic variability in RDH5 retinopathy (Fundus Albipunctatus). Ophthalmol 118, 1661-1670.

Grönemeyer JL, Sofía Burbano C, Hurek T,  Reinhold-Hurek B (2011) Isolation and characterization of root-associated bacteria from agricultural crops in the Kavango region of Namibia. Plant Soil (in press) DOI: 10.1007/s11104-011-0798-7.

Junker RR, Loewel C, Gross, R et al. (2011) Composition of epiphytic bacterial communities differs on petals and leaves. Plant Biol 13, 918–924.

Sergouniotis PI, Li Z, Mackay DS, Wright GA et al. (2011) A Survey of DNA Variation of C2ORF71 in Probands with Progressive Autosomal Recessive Retinal Degeneration and Control. IOVS 52, 1880-1886.

Türke M, Fiala B, Linsenmair KE, Feldhaar H (2010) Estimation of dispersal distances of the obligately plant-associated ant Crematogaster decamera. Ecol Entomol 35, 662-671.

Sandberger L, Feldhaar H, Lampert KP et al. (2010) Small, specialised and highly mobile? The tree-hole breeding frog, Phrynobatrachus guineensis, lacks fine-scale population structure. African J Herpetol 59, 79-94.

Müller JS, Jepson CD, Laval SH et al. (2010) Dok-7 promotes slow muscle integrity as well as neuromuscular junction formation in a zebrafish model of congenital myasthenic syndromes. Human Mol Genet (in press), doi: 10.1093/hmg/ddq049

Mukhopadhyay R, Sergouniotis PI, Mackay DS et al. (2010) A detailed phenotypic assessment of individuals affected by MFRP-related oculopathy. Mol Vis 16, 540-548.

Mackay DS, Henderson RH, Sergouniotis PI et al. (2010) Novel mutations in MERTK associated with childhood onset rodcone dystrophy. Mol Vis 16, 369-377.

Krebes L, Blank M, Jürss K, Zettler ML, Bastrop R (2010) Glacial-driven vicariance in the amphipod Gammarus duebeni. Mol Phylogen Evol 54, 372-385.

Götz A, Goebel W (2010) Glucose and glucose 6-phosphate as carbon sources in extra- and intracellular growth of enteroinvasive Escherichia coli and Salmonella enterica. Microbiology 156, 1176-1187.

Fuehrer HP, Blöschl I, Siehs C, Hassl A (2010) Detection of Toxoplasma gondii, Neospora caninum, and Encephalitozoon cuniculi in the brains of common voles (Microtus arvalis) and water voles (Arvicola terrestris) by gene amplification techniques in western Austria (Vorarlberg). Parasitol Res 107, 469-473.

Frankowski J, Bastrop R (2010) Identification of Anguilla anguilla (L.) and Anguilla rostrata (Le Sueur) and their hybrids based on a diagnostic single nucleotide polymorphism in nuclear 18S rDNA. Mol Ecol 10, 173-176.

Finger A, Schmitt T, Zachos FE, Meyer M, Assmann T, Habel JC (2009) The genetic status of the violet copper Lycaena helle – a relict of the cold past in times of global warming. Ecography 32, 382-390.

Stoll S, Feldhaar H, Gross R (2009) Transcriptional profiling of the endosymbiont Blochmannia floridanus during different developmental stages of its holometabolous ant host. Environ Microbiol 11, 877-888.

Thomas ML, Becker K, Abbott K, Feldhaar H (2009) Supercolony mosaics: two different invasions by the yellow crazy ant, Anoplolepis gracilipes, on Christmas Island, Indian Ocean Biol Invasions 12, 677-687.

Roiser JP, de Martino B, Tan GCY, Kumaran D, Seymour B, Wood NW, Dolan RJ (2009) A genetically driven bias in decision making driven by failure of amygdala control. J Neurosci 29, 5985-5991.

Frankowski J, Jennerich S, Schaarschmidt T, Ubl K, Jürss K, Bastrop R (2009) Validation of the occurrence of the American eel Anguilla rostrata (Le Sueur, 1817) in free-draining European inland waters. Biol Invasions 11, 1301-1309.

Habel JC, Zachos FE, Finger A, Meyer M, Louy D, Assmann T, Schmitt T (2009) Unprecedented long-term genetic monomorphisms in an endangered relict butterfly species. Conserv Genet 10, 1659-1665.

Blank M, Bastrop R (2008) Phylogeny of the mud worm genus Marenzelleria (Polychaeta, Spionidae) inferred from mitochondrial DNA sequences. Zoologica Scripta 38, 313-321.

Habel JC, Finger A, Meyer M, Schmitt T, Assmann T (2008) Polymorphic microsatellite loci in the endangered butterfly Lycaena helle (Lepidoptera: Lycaenidae). Eur J Entomol 105, 361–362.

Braumann I, van den Berg MA, Kempken K (2008) Strain-specific retrotransposon-mediated recombination in commercially used Aspergillus niger strain. Mol Gen Genom 280, 319-325.

Harms K, Wackernagel W (2008) The RecBCD and SbcCD DNases suppress homology-facilitated illegitimate recombination during natural transformation of Acinetobacter baylyi. Microbiology 154, 2437-2445.

Grouffaud S, van West P, Avrova AO, Birch PRJ, Whisson SC (2008) Plasmodium falciparum and Hyaloperonospora parasitica effector translocation motifs are functional in Phytophthora infestans. Microbiology 154, 3743-3751.

Dissanayake DR, Wijewardana TG, Gunawardena GA, Poxton IR (2008) Distribution of lipopolysaccharide core types among avian pathogenic Escherichia coli in relation to the major phylogenetic groups. Vet Microbiol 132, 355-363.

Hicks D, Lampe AK, Barresi R, Charlton R, Fiorillio C, Bonnemann CG, Hudson J, Sutton R, Lochmüller H, Straub V, Bushby K (2008) A refined diagnostic algorithm for Bethlem myopathy. Neurology 70, 1192-1199.

Sakuma A, Fukamachi H, Ito K, Ito Y, Takeuchi S, Takahashi S (2008) Loss of Runx3 affects ovulation and estrogen-induced endometrial cell proliferation in female mice. Mol Reprod Devel 75, 1653-1661.

Nuryanto A, Duryadi D, Soedharma D, Blohm D (2007) Molecular phylogeny of giant clams based on mitochondrial DNA cytochrome C oxidase I gene. HAYATI J Biosci 14, 162-166.

Harms K, Schön V, Kickstein E, Wackernagel W (2007) The RecJ DNase strongly suppresses genomic integration of short but not long foreign DNA fragments by homology-facilitated illegitimate recombination during transformation of Acinetobacter baylyi. Mol Microbiol 64, 691-702.

Wilting A, Buckley-Beason VA, Feldhaar H, Gadau J, O’brien SJ, Linsenmair KE (2007) Clouded leopard phylogeny revisited: support for species recognition and population division between Borneo and Sumatra. Front Zool 4, 15-25.

Drescher J, Blüthgen N, Feldhaar H (2007) Population structure and intraspecific aggression in the invasive ant species Anoplolepis gracilipes in Malaysian Borneo. Mol Ecol 16, 1453-1465.

Kickstein E, Harms K, Wackernagel W (2007) Deletions of recBCD or recD influence genetic transformation differently and are lethal together with a recJ deletion in Acinetobacter baylyi. Microbiology 153, 2259-2270.

Lam C, Harder T (2007) Marine microalgae affect abundance and community richness of bacterioplancton in close proximity. J Phycol 43, 874-881.

Rabe KS, Kiko K, Niemeyer CM (2007) Characterization of the peroxidase activity of CYP119, a thermostable P450 from Sulfolobus acidocaldarius. ChemBioCem 9, 420-425.

Harms K, de Vries J, Wackernagel W (2007) A double kill gene cassette for the positive selection of transforming non-selective DNA segments in Acinetobacter baylyi BD413. J Microbiol Meth 69,107-115.

Feldhaar H, Drescher J, Blüthgen N (2006). Characterization of microsatellite markers for the invasive ant species Anoplolepis gracilipes. Mol Ecol 6, 912-914.

 

References - DNA-free PCR Reagents

Junker RR, Romeike T, Keller A, Langen D (2014) Density-dependent negative responses by bumblebees to bacteria isolated from flowers. Apidologie 45, 467-477.

Harris KA, Yam T, Jalili S, Williams OM, Alshafi K, Gouliouris T, Munthali P, NiRiain U, Hartley JC (2014) Service evaluation to establish the sensitivity, specificity and additional value of broad-range 16S rDNA PCR for the diagnosis of infective endocarditis from resected endocardial material in patients from eight UK and Ireland hospitals. Eur J Clin Microbiol & Inf Dis, DOI 10.1007/s10096-014-2145-4.

Walsh AM, Sweeney T, O'Shea CJ, Doyle DN, O'Doherty JV (2013) Effect of supplementing varying inclusion levels of laminarin and fucoidan on growth performance, digestibility of diet components, selected faecal microbial populations and volatile fatty acid concentrations in weaned pigs. Animal Feed Sci Tech (in press) doi:10.1016/j.anifeedsci.2013.04.013.

Dubois D, Grare M, Prere MF et al. (2012) Performances of the Vitek MS matrix-assisted laser desorption ionization–time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology. J Clin Microbiol 50: 2568-2576.

Pekova S, Vydra J, Kabickova H, Frankova S, Haugvicova R, Mazal O, Cmejla R, Hardekopf DW, Jancuskova T, Kozak T (2011) Candidatus Neoehrlichia mikurensis infection identified in 2 hematooncologic patients: benefit of molecular techniques for rare pathogen detection. Diagn Microbiol Infect Dis 69, 266–270.

Peham JR, Grienauer W, Steiner H, Heer R, Vellekoop MJ, Nöhammer C, Wiesinger-Mayr H (2011) Long target droplet polymerase chain reaction with a microfluidic device for high-throughput detection of pathogenic bacteria at clinical sensitivity. Biomed Microdevices (in press); DOI 10.1007/s10544-011-9514-x.

Gerçe B, Schwartz T, Syldatk C, Hausmann R (2011) Differences between bacterial communities associated with the surface or tissue of mediterranean sponge species. Microb Ecol (in press) DOI: 10.1007/s00248-011-9802-2.

Mühl H, Kochem AJ, Disqué C, Sakka SG (2010) Activity and DNA contamination of commercial polymerase chain reaction reagents for the universal 16S rDNA real-time polymerase chain reaction detection of bacterial pathogens in blood. Diag. Microbiol. Infect. Dis. 66, 41-49.

Jones HE, Harris KA, Azizia M, Bank L, Carpenter B, Hartley JC, Klein N, Peebles D (2009) Differing Prevalence and Diversity of Bacterial Species in Fetal Membranes from Very Preterm and Term Labor. PLoS One 4, e8205; doi:10.1371/journal.pone.0008205.

Handschur M, Karlic H, Hertel C, Pfeilstöcker M, Haslberger AG (2009) Preanalytic removal of human DNA eliminates false signals in general 16S rDNA PCR monitoring of bacterial pathogens in blood. CIMID 32, 207-219.

 

Standard PCR Reagents

 
MolTaq  Benefits:
moltaq_pcrstrips_web72_10
  • Highly active Taq DNA polymerases
  • Suitable for Real-Time PCR
  • Ready-to-use mastermixes
  • Stained PCR reagents for safe pipetting and direct gel loading available
  • PCR enhancer for GC-rich and difficult templates

Molzym’s high quality products for daily routine PCRs comprise a variety of highly active Taq DNA polymerases. The products suit all applications of PCR and Real-Time PCR assays. MolTaq products serve in analyses of SNPs, human, animal and plant genetic markers, molecular epidemiology, molecular ecology as well as animal and plant breeding, food and water quality control, sequencing reactions and gene expression studies.

MolTaq is a highly processive, thermostable Taq DNA polymerase with 5’-3’ exonuclease activity useful for research and routine PCR. Products are available supplying buffer with or without magnesium (1.5mM final concentration). Red dye-containing products, MolTaqRED and MolTaqRED basic support easy gel loading and exclusion of pipetting errors by visualisation of the PCR solution.

 

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